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u6 expression vectors  (Addgene inc)


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    Structured Review

    Addgene inc u6 expression vectors
    U6 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript/SpCas9_sgRNA_expression_in_pBluescript+(Plasmid+%23122089)/pmc12957561-33-23-26
    Average 93 stars, based on 12 article reviews
    u6 expression vectors - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Optimal trade-off control in machine learning–based library design, with application to adeno-associated virus (AAV) for gene therapy
    Article Snippet: .. Specifically, in a ~75 to 80% confluent density of 15-cm dish of HEK 293 T cells, 13.5 μg of pHelper, 9 μg of pBluescript (Addgene), 70 ng of the capsid plasmid library, and 5 μg of pRepHelper were cotransfected by the polyethyleneimine (PEI) method. ..

    Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer
    Article Snippet: Mycoplasma testing is performed routinely during cell culture using MycoProbe Mycoplasma Detection Kit (R&D Systems, CUL001B; Minneapolis, MN, USA). .. Two plasmid component systems - Cas9 expressing plasmid pSpCas92AGFP (Addgene PX458, Plasmid 48138, RRID: Addgene_48138; Waltham, MA, USA) and pBluescript (RRID: Addgene_133885) U6 CHD1 Zg1 sgRNA were used. ..

    Article Title: Optimal trade-off control in machine learning-based library design, with application to adeno-associated virus (AAV) for gene therapy.
    Article Snippet: .. Specifically, in a ~75 to 80% confluent density of 15- cm dish of HEK 293 T cells, 13.5 μg of pHelper, 9 μg of pBluescript (Addgene), 70 ng of the capsid plasmid library, and 5 μg of pRepHelper were cotransfected by the polyethyleneimine (PEI) method. ..

    Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer.
    Article Snippet: Mycoplasma testing is performed routinely during cell culture using MycoProbe Mycoplasma Detection Kit (R&D Systems, CUL001B; Minneapolis, MN, USA). .. CHD1 knockout using CRISPR-Cas9 technology Two plasmid component systems - Cas9 expressing plasmid pSpCas92AGFP (Addgene PX458, Plasmid 48138, RRID: Addgene_48138; Waltham, MA, USA) and pBluescript (RRID: Addgene_133885) U6 CHD1 Zg1 sgRNA were used. ..

    Article Title: Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in Caenorhabditis elegans
    Article Snippet: .. The used injection mix was composed of GFP-expression plasmid (10 ng/μl), pRF4:rol-6(su1006) co-injection marker (50 ng/μl) [ ], and pBluescript (Addgene) empty plasmid (180 ng/μl) was added to reach a total DNA concentration of 100–200 ng/μl in sterile ddH2O. .. Agarose pads (2%) and Halocarbon oil 700 (Sigma-Aldrich) were used for immobilization, and a standard M9 medium was used as a recovery buffer [ ].

    Expressing:

    Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer
    Article Snippet: Mycoplasma testing is performed routinely during cell culture using MycoProbe Mycoplasma Detection Kit (R&D Systems, CUL001B; Minneapolis, MN, USA). .. Two plasmid component systems - Cas9 expressing plasmid pSpCas92AGFP (Addgene PX458, Plasmid 48138, RRID: Addgene_48138; Waltham, MA, USA) and pBluescript (RRID: Addgene_133885) U6 CHD1 Zg1 sgRNA were used. ..

    Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer.
    Article Snippet: Mycoplasma testing is performed routinely during cell culture using MycoProbe Mycoplasma Detection Kit (R&D Systems, CUL001B; Minneapolis, MN, USA). .. CHD1 knockout using CRISPR-Cas9 technology Two plasmid component systems - Cas9 expressing plasmid pSpCas92AGFP (Addgene PX458, Plasmid 48138, RRID: Addgene_48138; Waltham, MA, USA) and pBluescript (RRID: Addgene_133885) U6 CHD1 Zg1 sgRNA were used. ..

    Injection:

    Article Title: Distinct neurogenetic mechanisms establish the same chemosensory valence state at different life stages in Caenorhabditis elegans
    Article Snippet: .. For tissue-specific rescue of daf-2 , the following plasmids containing the daf-2 cDNA were obtained from Addgene and individually injected into the CB1370 daf-2 ( e1370 ) strain at 30 ng/μL, along with P myo-2 :: dsRed (20 ng/μL) as a coinjection marker and pBlueScript (50 ng/μL): pJH4531 (P rgef-1 :: daf-2 ) for pan-neuronal rescue (Addgene #132366), pJH4723 (P ges-1 :: daf-2 ) for intestinal rescue (Addgene #178899), and pJH4733 (P myo-3 :: daf-2 ) for muscle rescue (Addgene #178898) ( Hung et al. 2014 ). ..

    Article Title: Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in Caenorhabditis elegans
    Article Snippet: .. The used injection mix was composed of GFP-expression plasmid (10 ng/μl), pRF4:rol-6(su1006) co-injection marker (50 ng/μl) [ ], and pBluescript (Addgene) empty plasmid (180 ng/μl) was added to reach a total DNA concentration of 100–200 ng/μl in sterile ddH2O. .. Agarose pads (2%) and Halocarbon oil 700 (Sigma-Aldrich) were used for immobilization, and a standard M9 medium was used as a recovery buffer [ ].

    Marker:

    Article Title: Distinct neurogenetic mechanisms establish the same chemosensory valence state at different life stages in Caenorhabditis elegans
    Article Snippet: .. For tissue-specific rescue of daf-2 , the following plasmids containing the daf-2 cDNA were obtained from Addgene and individually injected into the CB1370 daf-2 ( e1370 ) strain at 30 ng/μL, along with P myo-2 :: dsRed (20 ng/μL) as a coinjection marker and pBlueScript (50 ng/μL): pJH4531 (P rgef-1 :: daf-2 ) for pan-neuronal rescue (Addgene #132366), pJH4723 (P ges-1 :: daf-2 ) for intestinal rescue (Addgene #178899), and pJH4733 (P myo-3 :: daf-2 ) for muscle rescue (Addgene #178898) ( Hung et al. 2014 ). ..

    Article Title: Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in Caenorhabditis elegans
    Article Snippet: .. The used injection mix was composed of GFP-expression plasmid (10 ng/μl), pRF4:rol-6(su1006) co-injection marker (50 ng/μl) [ ], and pBluescript (Addgene) empty plasmid (180 ng/μl) was added to reach a total DNA concentration of 100–200 ng/μl in sterile ddH2O. .. Agarose pads (2%) and Halocarbon oil 700 (Sigma-Aldrich) were used for immobilization, and a standard M9 medium was used as a recovery buffer [ ].

    other:

    Article Title: Microbiota dictate T cell clonal selection to augment graft-versus-host disease after stem cell transplantation.
    Article Snippet: In brief Allogeneic T cell expansion is the primary determinant of graft-versus-host disease (GVHD), and current dogma dictates that this is driven by histocompatibility antigen disparities between donor and recipient.. Yeh et al. found that a subset of donor CD4 T cell clonal expansion was microbiota dependent and that microbiota-specific T cells augmented GVHD lethality independent of donorrecipient genetics.

    Knock-Out:

    Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer.
    Article Snippet: Mycoplasma testing is performed routinely during cell culture using MycoProbe Mycoplasma Detection Kit (R&D Systems, CUL001B; Minneapolis, MN, USA). .. CHD1 knockout using CRISPR-Cas9 technology Two plasmid component systems - Cas9 expressing plasmid pSpCas92AGFP (Addgene PX458, Plasmid 48138, RRID: Addgene_48138; Waltham, MA, USA) and pBluescript (RRID: Addgene_133885) U6 CHD1 Zg1 sgRNA were used. ..

    CRISPR:

    Article Title: Genetic loss of CHD1 regulates distinct histone post-translational modifications in the development of castration-resistant prostate cancer.
    Article Snippet: Mycoplasma testing is performed routinely during cell culture using MycoProbe Mycoplasma Detection Kit (R&D Systems, CUL001B; Minneapolis, MN, USA). .. CHD1 knockout using CRISPR-Cas9 technology Two plasmid component systems - Cas9 expressing plasmid pSpCas92AGFP (Addgene PX458, Plasmid 48138, RRID: Addgene_48138; Waltham, MA, USA) and pBluescript (RRID: Addgene_133885) U6 CHD1 Zg1 sgRNA were used. ..

    Concentration Assay:

    Article Title: Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in Caenorhabditis elegans
    Article Snippet: .. The used injection mix was composed of GFP-expression plasmid (10 ng/μl), pRF4:rol-6(su1006) co-injection marker (50 ng/μl) [ ], and pBluescript (Addgene) empty plasmid (180 ng/μl) was added to reach a total DNA concentration of 100–200 ng/μl in sterile ddH2O. .. Agarose pads (2%) and Halocarbon oil 700 (Sigma-Aldrich) were used for immobilization, and a standard M9 medium was used as a recovery buffer [ ].

    Sterility:

    Article Title: Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in Caenorhabditis elegans
    Article Snippet: .. The used injection mix was composed of GFP-expression plasmid (10 ng/μl), pRF4:rol-6(su1006) co-injection marker (50 ng/μl) [ ], and pBluescript (Addgene) empty plasmid (180 ng/μl) was added to reach a total DNA concentration of 100–200 ng/μl in sterile ddH2O. .. Agarose pads (2%) and Halocarbon oil 700 (Sigma-Aldrich) were used for immobilization, and a standard M9 medium was used as a recovery buffer [ ].

    Derivative Assay:

    Article Title: Nucleosomes represent a crucial target for the intra-S phase checkpoint in response to replication stress
    Article Snippet: .. The plasmids used for gene editing experiments were derived from pBluescript, pFA6a-kanMX6, and pSLF1072 as described elsewhere (Addgene, Watertown, MA, USA). .. Site-directed mutations of brl2 gene were introduced using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA), following the protocol provided by the manufacturer.



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    Addgene inc pbluescript ii ks p21 promoter luc plasmid
    LJ3402 prevents hepatic senescence and mitochondrial dysfunction in aged mice. (A) Representative images of hematoxylin and eosin (H&E) (top), SA‐β‐gal (middle), and Oil Red O (bottom) staining on frozen liver sections from the indicated group. (B) The mRNA levels of marker genes involved in senescence ( p16 , <t>p21</t> , p53 ) and protein levels (γH2AX, p21, p53) in the liver of young and aged mice were determined by RT‐qPCR or immunoblotting ( n = 3; three randomly selected samples per group). (C) Expression of marker genes involved in lipid metabolism ( Srebp1c , Pparα , Cpt1 ) and mitochondrial homeostasis ( Sirt1 , Pgc‐1α , NRF1 , TFAM ) in the liver was determined using RT‐qPCR. (D) Hepatic triglyceride (TG), total cholesterol (TC), and free fatty acid (FFA), and (E) plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ( n = 5; three randomly selected samples per group). (F) Livers separated from mice were incubated in culture medium, and the media were collected after 12 h and 24 h of incubation; the concentrations of TNF‐α and IL‐1β in the media were measured ( n = 5; three randomly selected samples per group). All data are presented as the mean ± SEM. * p < 0.05 and ** p < 0.01. Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05).
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    Addgene inc pbluescript sk
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    Image Search Results


    LJ3402 prevents hepatic senescence and mitochondrial dysfunction in aged mice. (A) Representative images of hematoxylin and eosin (H&E) (top), SA‐β‐gal (middle), and Oil Red O (bottom) staining on frozen liver sections from the indicated group. (B) The mRNA levels of marker genes involved in senescence ( p16 , p21 , p53 ) and protein levels (γH2AX, p21, p53) in the liver of young and aged mice were determined by RT‐qPCR or immunoblotting ( n = 3; three randomly selected samples per group). (C) Expression of marker genes involved in lipid metabolism ( Srebp1c , Pparα , Cpt1 ) and mitochondrial homeostasis ( Sirt1 , Pgc‐1α , NRF1 , TFAM ) in the liver was determined using RT‐qPCR. (D) Hepatic triglyceride (TG), total cholesterol (TC), and free fatty acid (FFA), and (E) plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ( n = 5; three randomly selected samples per group). (F) Livers separated from mice were incubated in culture medium, and the media were collected after 12 h and 24 h of incubation; the concentrations of TNF‐α and IL‐1β in the media were measured ( n = 5; three randomly selected samples per group). All data are presented as the mean ± SEM. * p < 0.05 and ** p < 0.01. Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05).

    Journal: Biofactors (Oxford, England)

    Article Title: Lactobacillus johnsonii JNU3402 Ameliorates Age‐Related Liver Dysfunction Through Stimulating PGC ‐1α‐Mediated SIRT1 Expression

    doi: 10.1002/biof.70069

    Figure Lengend Snippet: LJ3402 prevents hepatic senescence and mitochondrial dysfunction in aged mice. (A) Representative images of hematoxylin and eosin (H&E) (top), SA‐β‐gal (middle), and Oil Red O (bottom) staining on frozen liver sections from the indicated group. (B) The mRNA levels of marker genes involved in senescence ( p16 , p21 , p53 ) and protein levels (γH2AX, p21, p53) in the liver of young and aged mice were determined by RT‐qPCR or immunoblotting ( n = 3; three randomly selected samples per group). (C) Expression of marker genes involved in lipid metabolism ( Srebp1c , Pparα , Cpt1 ) and mitochondrial homeostasis ( Sirt1 , Pgc‐1α , NRF1 , TFAM ) in the liver was determined using RT‐qPCR. (D) Hepatic triglyceride (TG), total cholesterol (TC), and free fatty acid (FFA), and (E) plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ( n = 5; three randomly selected samples per group). (F) Livers separated from mice were incubated in culture medium, and the media were collected after 12 h and 24 h of incubation; the concentrations of TNF‐α and IL‐1β in the media were measured ( n = 5; three randomly selected samples per group). All data are presented as the mean ± SEM. * p < 0.05 and ** p < 0.01. Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05).

    Article Snippet: The pBluescript II KS(+)‐p21 promoter‐luc plasmid was purchased from Addgene (MA, USA) and designated as p21 promoter‐luc.

    Techniques: Staining, Marker, Quantitative RT-PCR, Western Blot, Expressing, Clinical Proteomics, Incubation

    SIRT1 modulates LJ3402‐mediated suppression of p53 function in senescent AML12 hepatocytes. (A) Acetylation of p53 after 5 days of treatment with LJ3402‐CM (LJ‐CM) and 50 μM sirtinol during H 2 O 2 ‐induced senescence. (A, C–I) Senescence of AML12 hepatocytes was induced by 1 mM or 750 μM H 2 O 2 for 1 h per day for 7 days. LJ‐CM, 50 μM sirtinol, or 20 μM PFTα were added on days 3–7 during H 2 O 2 exposure. (B) Luciferase reporter activity in HEK293T cells transfected with a reporter plasmid ( p21 promoter–luc) and a p53 expression plasmid. Twelve hours after transfection, cells were treated with LJ‐CM and 50 μM sirtinol for 24 h with or without 100 μM H 2 O 2 . (C) The mRNA and protein levels of p21, p53, PGC‐1α, and SIRT1 measured by RT‐qPCR and immunoblotting, respectively. (D) SA‐β‐gal staining. (E) mRNA levels of genes involved in mitochondrial biogenesis ( NRF1 and TFAM ) and oxidant defense ( CAT and SOD ). (F) ROS levels. (G) Oxygen consumption rates (OCRs) measured at baseline and after treatment with 2.5 μM oligomycin, 1 μM FCCP, or 1 μM rotenone/antimycin A. (H) Oil Red O staining. (I) Intracellular TG levels in senescent AML12 hepatocytes treated with LJ‐CM, sirtinol, and PFTα. All data are presented as the mean ± SEM. Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05).

    Journal: Biofactors (Oxford, England)

    Article Title: Lactobacillus johnsonii JNU3402 Ameliorates Age‐Related Liver Dysfunction Through Stimulating PGC ‐1α‐Mediated SIRT1 Expression

    doi: 10.1002/biof.70069

    Figure Lengend Snippet: SIRT1 modulates LJ3402‐mediated suppression of p53 function in senescent AML12 hepatocytes. (A) Acetylation of p53 after 5 days of treatment with LJ3402‐CM (LJ‐CM) and 50 μM sirtinol during H 2 O 2 ‐induced senescence. (A, C–I) Senescence of AML12 hepatocytes was induced by 1 mM or 750 μM H 2 O 2 for 1 h per day for 7 days. LJ‐CM, 50 μM sirtinol, or 20 μM PFTα were added on days 3–7 during H 2 O 2 exposure. (B) Luciferase reporter activity in HEK293T cells transfected with a reporter plasmid ( p21 promoter–luc) and a p53 expression plasmid. Twelve hours after transfection, cells were treated with LJ‐CM and 50 μM sirtinol for 24 h with or without 100 μM H 2 O 2 . (C) The mRNA and protein levels of p21, p53, PGC‐1α, and SIRT1 measured by RT‐qPCR and immunoblotting, respectively. (D) SA‐β‐gal staining. (E) mRNA levels of genes involved in mitochondrial biogenesis ( NRF1 and TFAM ) and oxidant defense ( CAT and SOD ). (F) ROS levels. (G) Oxygen consumption rates (OCRs) measured at baseline and after treatment with 2.5 μM oligomycin, 1 μM FCCP, or 1 μM rotenone/antimycin A. (H) Oil Red O staining. (I) Intracellular TG levels in senescent AML12 hepatocytes treated with LJ‐CM, sirtinol, and PFTα. All data are presented as the mean ± SEM. Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05).

    Article Snippet: The pBluescript II KS(+)‐p21 promoter‐luc plasmid was purchased from Addgene (MA, USA) and designated as p21 promoter‐luc.

    Techniques: Luciferase, Activity Assay, Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Western Blot, Staining